Short Answer

It depends on the level of your metagenome assembly.

Detailed Answer

Have a look at the ENA documentation to get to know the metagenome assembly levels.

When submitting Metagenome-Assembled Genome (MAGs), you would need:

1. a LOCUS TAG of your choice
2. an EMBL file
3. the values that are usually asked for primary assemblies (raw version of the final assembly), which are:

• • COVERAGE: The estimated depth of sequencing coverage
  • PROGRAM: The assembly program
  • PLATFORM: The sequencing platform
  • MINGAPLENGTH: Minimum length of consecutive Ns to be considered a gap (optional)
  • MOLECULETYPE: ‘genomic DNA’, ‘genomic RNA’ or ‘viral cRNA’ (optional)
  • DESCRIPTION: Free text description of the genome assembly (optional)
  • RUN_REF: Comma separated list of run accession(s) (optional)

 If you have doubts about the name to give to your MAGs, have a look here.