Short Answer
Initially, you should provide the minimal information required.
Detailed Answer
The minimal information is:
- ASSEMBLYNAME: Unique assembly name
- COVERAGE: The estimated depth of sequencing coverage
- PROGRAM: The assembly program
Optional information includes:
- MINGAPLENGTH: Minimum length of consecutive Ns to be considered a gap
- MOLECULETYPE: ‘genomic DNA’, ‘genomic RNA’ or ‘viral cRNA’
- DESCRIPTION: Free text description of the genome assembly
Further information to the respective ENA docs here.