Short Answer

It depends on the level of your metagenome assembly.

Detailed Answer

Have a look at the ENA documentation to get to know the metagenome assembly levels.

When submitting Metagenome-Assembled Genome (MAGs), you would need:

  1. a LOCUS TAG of your choice
  2. an EMBL file
  3. the values that are usually asked for primary assemblies (raw version of the final assembly), which are:
    • COVERAGE: The estimated depth of sequencing coverage
    • PROGRAM: The assembly program
    • PLATFORM: The sequencing platform
    • MINGAPLENGTH: Minimum length of consecutive Ns to be considered a gap (optional)
    • MOLECULETYPE: ‘genomic DNA’, ‘genomic RNA’ or ‘viral cRNA’ (optional)
    • DESCRIPTION: Free text description of the genome assembly (optional)
    • RUN_REF: Comma separated list of run accession(s) (optional)

 If you have doubts about the name to give to your MAGs, have a look here.